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In vitro inhalation

Experienced team enabled by purpose-built facilities
  • Over 60 years' experience performing inhalation studies to recognized worldwide standards on a full range of agrochemicals, including difficult-to-handle, unpredictable, and hazardous materials, as well as nanomaterials.

  • A reputation for innovation in study design and test delivery mechanisms that optimizes the success of your inhalation toxicology study.

  • Dedicated teams of inhalation toxicology experts including aerosol technicians, specialist animal technicians, engineers, study managers, and pathologists.

Cytotoxiticty and mutagenicity assays

We offer a range of in vitro cytotoxicity and mutagenicity assays for testing on aerosolized compounds. Using specialist equipment, we can generate smoke or vapor from aerosol products for testing either in aerosol form or as total particulate matter (TPM)/aerosol collected matter or gas vapor phase (GVP) for testing as liquids. Our purpose-built gas testing chamber allows us to test gaseous compounds either neat or in dilution with compressed air directly onto the test system. We also have the capability to generate liquid aerosol from liquids and suspensions using the Vitrocell® Cloud system and test aerosols from powders with an RGB generator, enabling a more relevant testing environment.

Our team has extensive experience utilizing 3D tissue models both to GLP or with a research and development approach for novel endpoints. GLP methods include testing for cytotoxicity and oxidative stress. Listed below are the most common assays we run:

Services and TestsAerosolTPM/GVP
Ames test****
Micronucleus***
Comet***
2D cytotoxicity (NRU) – Balb/c 3t3, A549, CHO, H292 (others available on request)****
3D tissue cytotoxicity (MTT, WST-8)****
3D tissue oxidative Stress** 

*Non-GLP; **GLP

Supplementary endpoints for 3D models and our experience with the leading providers include:

Services and TestsMatTekEpithelix
Transepithelial electrical resistance (TEER)EpiAirway™, EpiOral™MucilAir™
Cilia beat frequency (CBF)EpiAirway™MucilAir™
Mucociliary clearanceEpiAirway™MucilAir™
Goblet cell hyperplasia (GCH)EpiAirway™MucilAir™
Apoptosis (histology)EpiAirway™, EpiOral™MucilAir™
Cytokine releaseEpiAirway™, EpiOral™MucilAir™
RNA expression analysisEpiAirway™, EpiOral™MucilAir™

Click to access crop protection and chemical in vitro test methods.

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